Phase I – Immunization
You have the choice between Dromedary and Lama. The dromedary provides more sequence variability and better stability.
Standard immunization (day 1/21/42/63/73) or Rapid immunization (day 1/7/14/21/28).
Phase II – Lymphocyte isolation, cDNA preparation, Amplification and phage library
cDNA is generated via RNA isolation, amplified via PCR, and a diverse phage library is generated for screening against the antigen.
Phase III – Intrabody Screening: Yeast two-hybrid
Yeast two-hybrid is an in vivo tool for the detection of direct protein-protein interactions. With features like the ability to form protein tertiary structures, neutral internal pH and a favoured environment for the formation of disulfide bonds, yeast is the ideal host to screen for intrabodies. With a library-based approach, screening for a suitable intrabody is possible in a high-throughput manner. This offers the possibility to find a set of different but highly specific intrabodies.
Download our documentation: Flyer Intrabodies 2.0-1
The phage library will be screened against the desired antigen and ideally 6 potential clones will be identified and evaluated. Our antibody fragments can be available for sale in small amount.
Phase IV – Production
We express the final antibody in a protein expression system. The desired quantity of antibody is produced and purified.
- We only charge a fee in case of success.
- In the case of unit non-response, we will re-inject the antigen (no additional charges).
- You are the only owner of intellectual property