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VHH LIBRARY GENERATION

solutions for your innovative research

Phase I – Immunization

You have the choixe between Dromedary and Lama. The dromedary provides more sequence variability and better stability.

Standard immunization (day 1/21/42/63/73) or Rapid immunization (day 1/7/14/21/28).

Phase II – Lymphocyte isolation, cDNA preparation, Amplification and phage library

cDNA is generated via RNA isolation, amplified via PCR, and a diverse phage library is generated for screening against the antigen.